Human Diagnostics
- Blood & Cell
- Quick Plasmid
- Universal Pathogen
- Viral RNA
Blood and Cell DNA Extraction
The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, ie. DNA extraction from blood and cells, as well as cell suspensions. DNA extraction from blood and cells is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.
Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of gDNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.
More Info
- High DNA recovery and quality of varying sizes
- DNA extraction from white blood cells
- Short and scalable protocol
- Cost effective extractions
- Rapid and cost-effective extractions
- Non-toxic chemicals
- Automatable on open liquid handling systems
Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.
- Performance
- Specifications
- Documentation
The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. A high quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.
The magnetiQ Blood & Cell DNA Extraction Kit also provides cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high-yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.
-Extraction yield of gDNA from 100 uL of whole blood using Galenvs magnetiQ Blood/Cell kit and Qiagen DNeasy Blood & Tissue kit. Quantification of gDNA performed using Qubit BR DNA fluorescent assay measurement (n=3)-
-Gel electrophoresis of extracted gDNA from 100 uL whole blood samples (4x) comparing Galenvs and Qiagen kits. Galenvs isolated gDNA is higher molecular weight while Qiagen extraction shows DNA smears below gDNA bands-
Download magnetiQ Blood & Cell Extraction Kit Technical Specifications
Applications
- Next Generation Sequencing (NGS)
- PCR and qPCR gene amplification
- DNA methylation studies
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates organic solvent hazardous waste
- Requires only the use of a magnetic rack and standard pipettes
Blood & Cell DNA Extraction
The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
Sample Type
Formats
Elution Volume
DNA recovered
Processing Mode
Throughput
Binding Technology
Binding Capacity
Components
Storage
Blood, Cells, Cell culture
Manual: 100 or 250 assays
Prefilled plates: 16 or 96 assays
100 µL
>98%
Automated; Manual. Compatible with 96-well plates.
1-250 samples per run
Magnetic beads
Scalable
(i) Lysis/Binding Buffer
(ii) Wash Buffer #1
(iii) Wash Buffer #2
(iv) Elution Buffer
(v) Proteinase K
Room temperature
Kit Size
100 preps
250 preps
16 preps ready-to-use prefilled plates
Product Code
BC0100
BC0250
BC0016
Quick Plasmid Miniprep
The magnetiQ Quick Plasmid Miniprep Kit enables a rapid and efficient method of plasmid extraction and purification without need for RNase supplement. The plasmid isolation kit provides extraction of plasmid supercoiled DNA through single-step magnetic bead-based lysis and capture of plasmids from transformed bacteria.
The magnetiQ Quick Plasmid Isolation Kit enables simple and rapid plasmid extraction from cultured bacteria in 15-20 minutes using magnetic nanoparticles. No need for spin columns and multiple centrifugation steps. Isolate highly pure concentrations of plasmid DNA with negligible gDNA, protein or endotoxin carryover.
More Info
- Plasmid isolation kit for high plasmid recovery without RNase
- Short and scalable protocol for both low and high-copy vectors
- Cost effective extractions
- Non-toxic chemicals
- Automatable on open systems
Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.
- Performance
- Specifications
- Documentation
Plasmid Miniprep
The magnetiQ Quick Plasmid Isolation Kit alleviates the need for centrifugation steps and separate collection tubes – in contrast to standard column-based kits. magnetiQ Quick Plasmid Miniprep Kit allows for facile bacterial lysis, bacterial plasmid binding, and endotoxin removal only using a standard magnetic rack. The purified bacterial plasmid DNA can be concentrated in volumes as low as 25 μL, compared to column-based kits which often necessitate larger elution volumes. The magnetiQ Quick Plasmid Miniprep Kit performance demonstrates high yield and pure plasmid DNA extraction for both low and high copy vectors. Sample processing is completed in less than 20 minutes, yielding high purity and efficient recovery of molecular biology grade plasmids.
Applications
• Molecular biology
• In Vitro transfection
• PCR, DNA sequencing, cloning
Features
• Amenable to high-throughput methods, including automation
• Faster and simpler magnetic collection and resuspension steps
• Reduces concern for clogging
• Eliminates RNase treatment
• Eliminates centrifugation steps with bacterial lysis and removal of cell debris, proteins, and denatured gDNA
Plasmid Miniprep
The magnetiQ Quick Plasmid Isolation Kit contains optimized buffers for molecular biology applications developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
Sample Type
Formats
Elution Volume
DNA recovered
Processing Mode
Throughput
Binding Technology
Binding Capacity
Components
Storage
Bacterial cell culture
Manual: 100, or 200 assays
Automatd: 16 or 96 assays
100 µL
>98%
Automated; Manual. Compatible with 96-well plates.
1-250 samples per run
Magnetic beads
Scalable
(i) GPL-1,
(ii) GPL-2,
(iii) Lysis/binding buffer,
(iv) Binding Magnetic Nanoparticles,
(v) Wash Buffer #1,
(vi) Wash Buffer #2,
(vii) Elution buffer(i) Binding Buffer
(ii) Wash Buffer
(iii) Elution Buffer
Room temperature
Viral RNA Extraction
The magnetiQ Viral RNA Extraction Kit enables a rapid and efficient method of extraction and purification of viral RNA. Purification of viral RNA is achieved through magnetic bead-based capture and subsequent elution into clean buffer.
One-step magnetic bead-based extraction and purification of viral RNA under 15 minutes from:
- Swab solutions (viral transport medium – VTM, and inactivation transport medium – ITM)
- Saliva samples
- Cell-free body fluids
- Bronchoalveolar lavage fluid
The magnetiQ Viral RNA Extraction Kit purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples. Obtain purified and high concentrations of RNA free of debris, salt, lipid and protein contamination.
More Info
- High viral RNA recovery and quality of varying sizes
- Short and scalable protocol
- Rapid and cost-effective extractions
- Non-toxic chemicals
- Automatable on open systems
Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.
- Performance
- Specifications
- Documentation
Viral RNA Extraction
The magnetiQ Viral RNA Extraction Kit alleviates the need for centrifugation steps and separate collection tubes – in contrast to standard column-based kits. The magnetiQ Viral RNA Extraction capture efficiency is >99% of viral RNA. The purified sample can be concentrated in volumes as low as 25 μL, compared to column-based kits which often necessitate larger elution volumes. The magnetiQ Viral RNA Extraction Kit performance demonstrates high purification efficacy at various fragment sizes. Sample processing is completed in less than 15 minutes, yielding high purity and efficient recovery of viral RNA.
See Full Comparison – Galenvs/Norgen/Zymo Viral RNA Extraction Kits
Applications
•
- Diagnostics (IVDD) of COVID-19 in clinical laboratories for the isolation and recovery of SARS-CoV-2 RNA from patient samples
- Quantification via Reverse Transcriptase qPCR (RT-qPCR).
- Next Generation Sequencing (NGS)
- Genomic DNA library preparation
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging.
- Eliminates need for proteinase K treatment of swab samples
- Eliminates centrifugation steps associated with spin-column based viral extraction kits
Viral RNA Extraction
The magnetiQ Viral RNA Extraction Kit contains optimized buffers for viral RNA capture and purification developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
Sample Type
Formats
Elution Volume
DNA recovered
Processing Mode
Throughput
Binding Technology
Binding Capacity
Components
Storage
Swab, Saliva, Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), and swab preservative fluid, cell-free body fluids, , culture supernatants, bronchoalveolar lavage fluid
Manual: 20, 200,250 assays
Prefilled plates: 16 or 96 assays
25 µL or above
>99%
Automated; Manual. Compatible with 96-well plates.
1-250 samples per run
Magnetic beads
Scalable
(i) Viral Lysis/Binding Buffer
(ii) Wash Buffer #1
(iii) Wash Buffer #2
(iv) Elution Buffer
Room temperature
Kit Size
100 preps
250 preps
16 preps ready-to-use prefilled plates
96 preps ready-to-use prefilled plates
Product Code
VR0100
VR0250
VR0016
VR0096