Blood and Cell DNA Extraction Kit

The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, ie. DNA extraction from blood and cells, as well as cell suspensions. DNA extraction from blood and cells is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.

Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of gDNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.

High DNA recovery and quality of varying sizes

DNA extraction from white blood cells

Short and scalable protocol

Rapid and cost-effective extractions

Non-toxic chemicals

Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

Available in manual extraction formats

Also available in prefilled plate formats for automated extraction of blood and cell DNA

The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. A high quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.

The magnetiQ Blood & Cell DNA Extraction Kit also provides cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high-yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.

Extraction yield of gDNA from 100 uL of whole blood using Galenvs magnetiQ Blood/Cell kit and Qiagen DNeasy Blood & Tissue kit. Quantification of gDNA performed using Qubit BR DNA fluorescent assay measurement (n=3).

Gel electrophoresis of extracted gDNA from 100 uL whole blood samples (4x) comparing Galenvs and Qiagen kits. Galenvs isolated gDNA is higher molecular weight while Qiagen extraction shows DNA smears below gDNA bands.

Applications

  • Next Generation Sequencing (NGS)
  • PCR and qPCR gene amplification
  • DNA methylation studies
  • Copy number variation (CNV) studies

Features

  • Amenable to high-throughput methods, including automation
  • Faster and simpler magnetic collection and resuspension steps
  • Reduces concern for clogging
  • Eliminates organic solvent hazardous waste
  • Requires only the use of a magnetic rack and standard pipettes

Blood & Cell DNA Extraction Kit

The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.

Sample TypeBlood, Cells, Cell culture
FormatsManual: 100 or 250 assays
Prefilled plates: 16 or 96 assays
Elution Volume100 µL
DNA recovered>98%
Processing ModeAutomated; Manual. Compatible with 96-well plates.
Throughput1-250 samples per run
Binding TechnologyMagnetic beads
Binding CapacityScalable
Components(i) Lysis/Binding Buffer
(ii) Wash Buffer #1
(iii) Wash Buffer #2
(iv) Elution Buffer
(v) Proteinase K
StorageRoom temperature
Kit SizeProduct Code
100 prepsBC0100
250 prepsBC0250
16 preps ready-to-use prefilled platesBC0016

Blood & Cell DNA Extraction Kit

Quick Plasmid Miniprep Kit

The magnetiQ Quick Plasmid Miniprep Kit enables a rapid and efficient method of plasmid extraction and purification without need for RNase supplement. The plasmid isolation kit provides extraction of plasmid supercoiled DNA through single-step magnetic bead-based lysis and capture of plasmids from transformed bacteria.

The magnetiQ Quick Plasmid Isolation Kit enables simple and rapid plasmid extraction from cultured bacteria in 15-20 minutes using magnetic nanoparticles. No need for spin columns and multiple centrifugation steps. Isolate highly pure concentrations of plasmid DNA with negligible gDNA, protein or endotoxin carryover.

Plasmid isolation kit for high plasmid recovery without RNase

Short and scalable protocol for both low and high-copy vectors

Rapid and cost-effective extractions

Non-toxic chemicals

Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

Quick Plasmid Mini Prep

Available in manual extraction format

Quick Plasmid mini Prep - Automated

Also available in prefilled plate formats for automated extraction of plasmids

Quick Plasmid Miniprep Kit

The magnetiQ Quick Plasmid Isolation Kit alleviates the need for centrifugation steps and separate collection tubes – in contrast to standard column-based kits. magnetiQ Quick Plasmid Miniprep Kit allows for facile bacterial lysis, bacterial plasmid binding, and endotoxin removal only using a standard magnetic rack. The purified bacterial plasmid DNA can be concentrated in volumes as low as 25 μL, compared to column-based kits which often necessitate larger elution volumes. The magnetiQ Quick Plasmid Miniprep Kit performance demonstrates high yield and pure plasmid DNA extraction for both low and high copy vectors. Sample processing is completed in less than 20 minutes, yielding high purity and efficient recovery of molecular biology grade plasmids.

Applications

  • Molecular biology
  • In Vitro transfection
  • PCR, DNA sequencing, cloning

Features

  • Amenable to high-throughput methods, including automation
  • Faster and simpler magnetic collection and resuspension steps
  • Reduces concern for clogging
  • Eliminates RNase treatment
  • Eliminates centrifugation steps with bacterial lysis and removal of cell debris, proteins, and denatured gDNA

Quick Plasmid Miniprep Kit

The magnetiQ Quick Plasmid Isolation Kit contains optimized buffers for molecular biology applications developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.

Sample TypeBacterial cell culture
FormatsManual: 100 or 200 assays
Prefilled plates: 16 or 96 assays
Elution Volume100 µL
DNA recovered>98%
Processing ModeAutomated; Manual. Compatible with 96-well plates.
Throughput1-250 samples per run
Binding TechnologyMagnetic beads
Binding CapacityScalable
Components(i) GPL-1,
(ii) GPL-2,
(iii) Lysis/binding buffer,
(iv) Binding Magnetic Nanoparticles,
(v) Wash Buffer  #1,
(vi) Wash Buffer  #2,
(vii) Elution buffer(i) Binding Buffer
(ii) Wash Buffer
(iii) Elution Buffer
StorageRoom temperature

Quick Plasmid Miniprep Kit

Universal Pathogen DNA/RNA Extraction Kit

The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.

One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:

The Pathogen Extraction Kit provides a safe, simple and reproducible method for obtaining purified nucleic acid products. These can be used for downstream applications such as PCR, RT-PCR and Next Generation Sequencing (NGS).

Pathogen purification for high DNA & RNA recovery and quality of varying sizes

Short and scalable protocol

Rapid and cost-effective extractions

Non-toxic chemicals

Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

Available in 50- and 200-prep manual extraction formats

Also available in prefilled plate formats for automated extraction of pathogen DNA and RNA

Universal Pathogen DNA / RNA Extraction Kit

The magnetiQ Universal Pathogen Extraction kit enables researchers to obtain a pure, contaminant free product. Its chemistry is optimized to lyse cells and wash away protein, lipid, salt and any other contaminants to yield a high purity sample. Our lysis buffer disrupts the components of the matrix enabling our magnetic beads to specifically target nucleic acids, which are subsequently purified from contaminants by the washing buffers and eluted in the final step of the process.  The Pathogen Extraction kit has been optimized for 200 µl of sample from which the DNA or RNA is eluted in 50-100 µl, however there is some flexibility in the protocol. Researchers have the capacity to use a range of sample from 100-400 µl and to increase the concentration of the product by eluting in volumes as low as 50 µl. DNA and RNA can be recovered from initial concentrations as low as 1 copy/µl.

The magnetic beads used in the lysis buffer were developed to bind to DNA as well as RNA of different sizes with high specificity, without the use of Carrier RNA. This eliminates the need for any centrifugation step, filters, requires a minimal amount of collection tubes and can be easily applied to high throughput applications. It does not require any additives such as Proteinase K or RNA carrier.

Galenvs vs Omega Universal Pathogen extraction kit
Galenvs vs Qiagen Universal Pathogen extraction kit

Applications

  • Extraction of COVID-19 in clinical laboratories for the isolation and recovery of SARS-CoV-2 RNA
  • Research and detection of viral and bacterial infection
  • Quantification via Reverse Transcriptase qPCR (RT-qPCR)
  • Next Generation Sequencing (NGS)
  • Genomic DNA library preparation
  • Copy number variation (CNV) studies

Features

  • Amenable to high-throughput methods, including automation
  • Faster and simpler magnetic collection and resuspension steps
  • Reduces concern for clogging
  • Eliminates need for Proteinase K treatment of swab samples
  • Eliminates need for Carrier RNA in all sample types
  • Eliminates centrifugation steps associated with spin-column based viral extraction kits

Universal Pathogen DNA / RNA Extraction Kit

The magnetiQ Pathogen Extraction Kit has buffers optimized for pathogenic RNA and DNA capture. The functionalized magnetic beads in the lysis buffer were developed to target and bind nucleic acids specifically. All the components are stored at room temperature.

Sample TypeSwab, Saliva, Serum / Plasma, Blood, Stool, Urine, and Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), Exudate, CSF
FormatsManual: 50 or 200 assays
Automated: 16 assays
Elution Volume50-100 µL
DNA recovered>99%
Processing ModeAutomated; Manual. Compatible with 96-well plates.
Throughput1-250 samples per run
Binding TechnologyMagnetic beads
Binding CapacityScalable
Components(i) Pathogen Lysis/Binding Buffer
(ii) Binding Magnetic Nanoparticles
(iii) Wash Buffer #1
(iv) Wash Buffer #2
(v) Elution Buffer
StorageRoom temperature
Kit SizeProduct Code
50 prepsUP0050
200 prepsUP0200
16 preps ready-to-use prefilled platesUP0016
96 preps ready-to-use prefilled platesUP0096