Animal Diagnostics

Universal Pathogen
Viral RNA

Universal Pathogen DNA / RNA Extraction Kit

Higher DNA/RNA From Any Pathogen/Sample Matrix

Fast 20 min Protocol

One-Step Magnetic Extractions and Purification

Automatable /High Throughput

The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.

One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:

Swab solutions (viral transport medium – VTM, and inactivation transport medium – ITM)
Serum/plasma
Blood and Exudate
Saliva samples
Cell-free body fluids
Complex matrices such as sputum or stool
Cerebrospinal fluid (CSF)

The Pathogen Extraction Kit provides a safe, simple and reproducible method for obtaining purified nucleic acid products. These can be used for downstream applications such as PCR, RT-PCR and Next Generation Sequencing (NGS).

More Info

Pathogen purification for high DNA & RNA recovery and quality of varying sizes
Short and scalable protocol
Rapid and cost-effective extractions
Non-toxic chemicals
Automatable on open systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated platforms.

No carrier RNA or Proteinase K required.

Available in prefilled plate formats for automated extraction of Pathogen DNA and RNA.

Performance
Specifications
Documentation

Universal Pathogen DNA / RNA Extraction Kit

The magnetiQ Pathogen Extraction kit enables researchers to obtain a pure, contaminant free product. Its chemistry is optimized to lyse cells and wash away protein, lipid, salt and any other contaminants to yield a high purity sample. Our lysis buffer disrupts the components of the matrix enabling our magnetic beads to specifically target nucleic acids, which are subsequently purified from contaminants by the washing buffers and eluted in the final step of the process. The Pathogen Extraction kit has been optimized for 200 µl of sample from which the DNA or RNA is eluted in 50-100 µl, however there is some flexibility in the protocol. Researchers have the capacity to use a range of sample from 100-400 µl and to increase the concentration of the product by eluting in volumes as low as 50 µl. DNA and RNA can be recovered from initial concentrations as low as 1 copy/µl.

The magnetic beads used in the lysis buffer were developed to bind to DNA as well as RNA of different sizes with high specificity, without the use of Carrier RNA. This eliminates the need for any centrifugation step, filters, requires a minimal amount of collection tubes and can be easily applied to high throughput applications. It does not require any additives such as proteinase K or RNA carrier.

Galenvs vs Omega Universal Pathogen extraction kit

Galenvs vs Qiagen Universal Pathogen extraction kit

Download Full Comparison with Industry-Leading Kits from Omega & Qiagen

Applications

Extraction of COVID-19 in clinical laboratories for the isolation and recovery of SARS-CoV-2 RNA
Research and detection of viral and bacterial infection
Quantification via Reverse Transcriptase qPCR (RT-qPCR)
Next Generation Sequencing (NGS)
Genomic DNA library preparation
Copy number variation (CNV) studies

Features

Amenable to high-throughput methods, including automation
Faster and simpler magnetic collection and resuspension steps
Reduces concern for clogging
Eliminates need for Proteinase K treatment of swab samples
Eliminates need for Carrier RNA in all sample types
Eliminates centrifugation steps associated with spin-column based viral extraction kits

Universal Pathogen DNA / RNA Extraction Kit

The magnetiQ Pathogen Extraction Kit has buffers optimized for pathogenic RNA and DNA capture. The functionalized magnetic beads in the lysis buffer were developed to target and bind nucleic acids specifically. All the components are stored at room temperature.

Sample Type

Formats

Elution Volume

DNA recovered

Processing Mode

Throughput

Binding Technology

Binding Capacity

Components

Storage

Swab, Saliva, Serum / Plasma, Blood, Stool, Urine, and Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), Exudate, CSF

Manual: 50 or 200 assays
Automated: 16 assays

50-100 µL

>99%

Automated; Manual. Compatible with 96-well plates.

1-250 samples per run

Magnetic beads

Scalable

(i) Pathogen Lysis/Binding Buffer
(ii) Binding Magnetic Nanoparticles
(iii) Wash Buffer #1
(iv) Wash Buffer #2
(v) Elution Buffer

Room temperature

Kit Size

50 preps

200 preps

16 preps ready-to-use prefilled plates

96 preps ready-to-use prefilled plates

Product Code

UP0050

UP0200

UP0016

UP0096

Universal Pathogen Extraction

GUIDES & PROTOCOLS

Technical Specifications and Comparison with Omega & Qiagen – Universal Pathogen Kit

Animal / Veterinary White-paper – Universal Pathogen Kit

Viral RNA Extraction

IVD Approval – Health Canada

Fast 15 min Protocol

One-Step Magnetic RNA Extraction and Purification

Automatable/High Throughput

The magnetiQ Viral RNA Extraction Kit enables a rapid and efficient method of extraction and purification of viral RNA. Purification of viral RNA is achieved through magnetic bead-based capture and subsequent elution into clean buffer.

One-step magnetic bead-based extraction and purification of viral RNA under 15 minutes from:

Swab solutions (viral transport medium – VTM, and inactivation transport medium – ITM)
Saliva samples
Cell-free body fluids
Bronchoalveolar lavage fluid

The magnetiQ Viral RNA Extraction Kit purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples. Obtain purified and high concentrations of RNA free of debris, salt, lipid and protein contamination.