Human Diagnostics
- Blood & Cell
- Universal Pathogen
- Quick Plasmid
- Viral RNA
Blood and Cell DNA Extraction
Higher gDNA Yield and Purity
Quick 20 min Protocol
Effortless Magnetic Extractions
Single-Step Lysis and Capture of DNA
The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, ie. DNA extraction from blood and cells, as well as cell suspensions. DNA extraction from blood and cells is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.
Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of gDNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.
More Info
- High DNA recovery and quality of varying sizes
- DNA extraction from white blood cells
- Short and scalable protocol
- Cost effective extractions
- Rapid and cost-effective extractions
- Non-toxic chemicals
- Automatable on open liquid handling systems
Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.
Available in prefilled plate formats for automated extraction of Blood and Cell DNA.
- Performance
- Specifications
- Documentation
The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. A high quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.
The magnetiQ Blood & Cell DNA Extraction Kit also provides cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high-yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.
-Extraction yield of gDNA from 100 uL of whole blood using Galenvs magnetiQ Blood/Cell kit and Qiagen DNeasy Blood & Tissue kit. Quantification of gDNA performed using Qubit BR DNA fluorescent assay measurement (n=3)-
-Gel electrophoresis of extracted gDNA from 100 uL whole blood samples (4x) comparing Galenvs and Qiagen kits. Galenvs isolated gDNA is higher molecular weight while Qiagen extraction shows DNA smears below gDNA bands-
Download magnetiQ Blood & Cell Extraction Kit Technical Specifications
Applications
- Next Generation Sequencing (NGS)
- PCR and qPCR gene amplification
- DNA methylation studies
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates organic solvent hazardous waste
- Requires only the use of a magnetic rack and standard pipettes
Blood & Cell DNA Extraction
The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.
Sample Type
Formats
Elution Volume
DNA recovered
Processing Mode
Throughput
Binding Technology
Binding Capacity
Components
Storage
Blood, Cells, Cell culture
Manual: 100 or 250 assays
Prefilled plates: 16 or 96 assays
100 µL
>98%
Automated; Manual. Compatible with 96-well plates.
1-250 samples per run
Magnetic beads
Scalable
(i) Lysis/Binding Buffer
(ii) Wash Buffer #1
(iii) Wash Buffer #2
(iv) Elution Buffer
(v) Proteinase K
Room temperature
Kit Size
100 preps
250 preps
16 preps ready-to-use prefilled plates
Product Code
BC0100
BC0250
BC0016
Universal Pathogen DNA / RNA Extraction Kit
Higher DNA/RNA From Any Pathogen/Sample Matrix
Fast 20 min Protocol
One-Step Magnetic Extractions and Purification
Automatable /High Throughput
The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.
One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:
- Swab solutions (viral transport medium – VTM, and inactivation transport medium – ITM)
- Serum/plasma
- Blood and Exudate
- Saliva samples
- Cell-free body fluids
- Complex matrices such as sputum or stool
- Cerebrospinal fluid (CSF)
The Pathogen Extraction Kit provides a safe, simple and reproducible method for obtaining purified nucleic acid products. These can be used for downstream applications such as PCR, RT-PCR and Next Generation Sequencing (NGS).
More Info
- Pathogen purification for high DNA & RNA recovery and quality of varying sizes
- Short and scalable protocol
- Rapid and cost-effective extractions
- Non-toxic chemicals
- Automatable on open systems
Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated platforms.
No carrier RNA or Proteinase K required.
Available in prefilled plate formats for automated extraction of Pathogen DNA and RNA.
- Performance
- Specifications
- Documentation
Universal Pathogen DNA / RNA Extraction Kit
The magnetiQ Pathogen Extraction kit enables researchers to obtain a pure, contaminant free product. Its chemistry is optimized to lyse cells and wash away protein, lipid, salt and any other contaminants to yield a high purity sample. Our lysis buffer disrupts the components of the matrix enabling our magnetic beads to specifically target nucleic acids, which are subsequently purified from contaminants by the washing buffers and eluted in the final step of the process. The Pathogen Extraction kit has been optimized for 200 µl of sample from which the DNA or RNA is eluted in 50-100 µl, however there is some flexibility in the protocol. Researchers have the capacity to use a range of sample from 100-400 µl and to increase the concentration of the product by eluting in volumes as low as 50 µl. DNA and RNA can be recovered from initial concentrations as low as 1 copy/µl.
The magnetic beads used in the lysis buffer were developed to bind to DNA as well as RNA of different sizes with high specificity, without the use of Carrier RNA. This eliminates the need for any centrifugation step, filters, requires a minimal amount of collection tubes and can be easily applied to high throughput applications. It does not require any additives such as proteinase K or RNA carrier.
Galenvs vs Omega Universal Pathogen extraction kit
Galenvs vs Qiagen Universal Pathogen extraction kit
Download Full Comparison with Industry-Leading Kits from Omega & Qiagen
Applications
- Extraction of COVID-19 in clinical laboratories for the isolation and recovery of SARS-CoV-2 RNA
- Research and detection of viral and bacterial infection
- Quantification via Reverse Transcriptase qPCR (RT-qPCR)
- Next Generation Sequencing (NGS)
- Genomic DNA library preparation
- Copy number variation (CNV) studies
Features
- Amenable to high-throughput methods, including automation
- Faster and simpler magnetic collection and resuspension steps
- Reduces concern for clogging
- Eliminates need for Proteinase K treatment of swab samples
- Eliminates need for Carrier RNA in all sample types
- Eliminates centrifugation steps associated with spin-column based viral extraction kits
Universal Pathogen DNA / RNA Extraction Kit
The magnetiQ Pathogen Extraction Kit has buffers optimized for pathogenic RNA and DNA capture. The functionalized magnetic beads in the lysis buffer were developed to target and bind nucleic acids specifically. All the components are stored at room temperature.
Sample Type
Formats
Elution Volume
DNA recovered
Processing Mode
Throughput
Binding Technology
Binding Capacity
Components
Storage
Swab, Saliva, Serum / Plasma, Blood, Stool, Urine, and Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), Exudate, CSF
Manual: 50 or 200 assays
Automated: 16 assays
50-100 µL
>99%
Automated; Manual. Compatible with 96-well plates.
1-250 samples per run
Magnetic beads
Scalable
(i) Pathogen Lysis/Binding Buffer
(ii) Binding Magnetic Nanoparticles
(iii) Wash Buffer #1
(iv) Wash Buffer #2
(v) Elution Buffer
Room temperature
Kit Size
50 preps
200 preps
16 preps ready-to-use prefilled plates
96 preps ready-to-use prefilled plates
Product Code
UP0050
UP0200
UP0016
UP0096