Blood and Cell DNA Extraction

Blood and Cell Extraction Kit
For Research Use Only.
  • High DNA recovery and quality of varying sizes
  • DNA extraction from white blood cells
  • Short and scalable protocol
  • Cost effective extractions
  • Rapid and cost-effective extractions
  • Non-toxic chemicals
  • Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

The magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of nucleic acid isolation from whole blood samples, ie. DNA extraction from blood and cells, as well as cell suspensions. DNA extraction from blood and cells is achieved through single-step magnetic bead-based lysis and capture of DNA from cells in blood, tissue, or cell culture samples.

Achieve highly efficient capture/elution of nucleic acids yielding purified solutions with minimal protein and salt contamination for downstream applications such as PCR and sequencing. Obtain purified and high concentrations of gDNA free of cell debris, salt, lipid and protein contamination in under 15 minutes.

The magnetiQ Blood & Cell DNA Extraction Kit alleviates the need for separate lysis and binding, heating, or centrifugation steps. The purified sample can be concentrated in volumes as low as 25 μL, in contrast to column-based kits which often necessitate larger elution volumes. The magnetiQ Blood & Cell DNA Extraction Kit displays superior performance in comparison to leading commercially available column-based kits. A high quantity of DNA is extracted from cell suspension using the magnetiQ Blood & Cell DNA Extraction Kit, resulting in a lower CT value for GAPDH gene.

The magnetiQ Blood & Cell DNA Extraction Kit also provides cleaner, less contaminated eluted nucleic acid preparations from 100 μL whole blood or cell suspension samples. The kit produces very pure and high-yield nucleic acid elute (ratio between 1.8-2.2) with high reproducibility.

See magnetiQ Blood & Cell Extraction Kit Technical Specifications 

Applications

  • Next Generation Sequencing (NGS)
  • PCR and qPCR gene amplification
  • DNA methylation studies
  • Copy number variation (CNV) studies

Features

  • Amenable to high-throughput methods, including automation
  • Faster and simpler magnetic collection and resuspension steps
  • Reduces concern for clogging
  • Eliminates organic solvent hazardous waste
  • Requires only the use of a magnetic rack and standard pipettes

  Blood & Cell DNA Extraction

The magnetiQ Blood & Cell DNA Extraction Kit contains optimized buffers for blood or cell lysis and DNA binding, washing and elution developed with Galenvs’ functionalised magnetic beads and optimized with machine-learning approaches for protocol development and reagent formulation.

Sample TypeBlood, Cells, Cell culture
FormatsManual: 100 or 250 assays
Automated: 16 or 96 assays
Elution Volume100 µL
DNA recovered>98%
Processing ModeAutomated; Manual. Compatible with 96-well plates.
Throughput1-250 samples per run
Binding TechnologyMagnetic beads
Binding CapacityScalable
Components(i) Lysis/Binding Buffer
(ii) Wash Buffer #1
(iii) Wash Buffer #2
(iv) Elution Buffer
(v) Proteinase K
StorageRoom temperature

DNA Extraction from Blood

DNA Extraction from Cells